A. Pour plate method
B. Spread plate method
C. Membrane filter method
D. MPN
I need to test efficacy of few preservative (Preservative with cosmetic products) against selected bacteria and fungi. So, I need a standard procedure for it. Thank you.
04 May 2019 6,012 4 View
I am dealing with Phosphate solubilizing bacteria. They produce different types of organic acids and secrete acid into medium. This acid can solubilize the complex phosphate compounds into...
02 March 2018 3,777 12 View
I am working with E.coli O157:H7 . I want to increase the yield of PCR product for DNA sequencing. So, I need a good protocol for plasmid DNA extraction from this strain. Any suggestions ? Thank...
02 March 2018 838 2 View
Introduction of exotic plants into an agricultural environment may disseminate and establish destructive plant pathogens that cause plant disease epidemics. How can I explain this using gene for...
31 December 2017 2,914 0 View
There is an evolutionary tendency to reduce the number of spores in the sporangium in the subdivision zygomycotina. What are the reasons for it?
31 December 2017 6,494 0 View
I am going to start a new research about N fixation bacteria. So, in these days i am studying researches regarding N fixing bacteria and Plant growth promoting bacteria. So, if anybody have...
31 December 2017 3,722 3 View
I did 16s rRNA sequencing for bacterial species and now I have 16s rRNA sequences of 4 isolated bacterial species. Can I upload that data into NCBI ? Can I get accession numbers for each bacteria?
11 December 2017 5,508 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...
30 July 2024 557 2 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,294 3 View