What is the standard procedure for Preservative efficacy test?

I need to test efficacy of few preservative (Preservative with cosmetic products) against selected bacteria and fungi. So, I need a standard procedure for it. Thank you.

04 May 2019 5,871 4 View

Phosphate solubilizing bacteria??????

I am dealing with Phosphate solubilizing bacteria. They produce different types of organic acids and secrete acid into medium. This acid can solubilize the complex phosphate compounds into...

02 March 2018 3,668 12 View

Can anyone recommend a procedure for plasmid extraction from E.coli which yields DNA suitable for DNA sequencing?

I am working with E.coli O157:H7 . I want to increase the yield of PCR product for DNA sequencing. So, I need a good protocol for plasmid DNA extraction from this strain. Any suggestions ? Thank...

02 March 2018 722 2 View

Gene for gene hypothesis?

Introduction of exotic plants into an agricultural environment may disseminate and establish destructive plant pathogens that cause plant disease epidemics. How can I explain this using gene for...

31 December 2017 2,780 0 View

Why does zygomycotina fungi sporangium contain less number of spores?

There is an evolutionary tendency to reduce the number of spores in the sporangium in the subdivision zygomycotina. What are the reasons for it?

31 December 2017 6,381 0 View

Does anybody have articles or informations regarding N fixation?

I am going to start a new research about N fixation bacteria. So, in these days i am studying researches regarding N fixing bacteria and Plant growth promoting bacteria. So, if anybody have...

31 December 2017 3,621 3 View

How shall I submit data into NCBI?

I did 16s rRNA sequencing for bacterial species and now I have 16s rRNA sequences of 4 isolated bacterial species. Can I upload that data into NCBI ? Can I get accession numbers for each bacteria?

11 December 2017 5,395 4 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View