What is the best method for dissolving non mulberry silk fibre?

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Could you please advise on the optimal amount of exopolysaccharide to extract from a 500 ml bacterial culture?

12 December 2023 9,480 0 View

Why does a cyclic voltammogram have multiple anodic or cathodic peaks for certain materials?

I took the CV of nickel ferrite using carbon black as the counter electrode, the voltammogram shows multiple reduction peaks. why does that happen?

21 September 2023 9,475 1 View

Why does the CV scan taken using different instruments show different results?

The CV plots of buffer solutions having PH 3-10 was done using CH instrument as well as using AutoLab. Both the results have different peak positions and different shapes.

21 September 2023 1,038 1 View

What kind of contamination is this?

I am working with U87MG cell line and I got contamination in one of the flasks. Can anyone tell me what type of contamination is this?

21 September 2023 8,669 0 View

How to identify alpha and beta cellulose on the basis of extraction method?

In my study we used chemical treatment for the extraction of cellulose.

17 August 2023 4,264 1 View

Free softwares for adsorption study its kinetics isotherm and thermodynamic and also which software is used for error data analysis?

In my study i am using nanocellulose based membrane for the adsorption study of dye and heavy metals

26 July 2023 2,307 4 View

Is there any postdoctoral position in foreign universities (outside India)?

Dear fellow researchers and professors, I am currently pursuing my PhD in the area of industrial management, and looking for postdoc in foreign universities outside India. If you are aware of some...

23 June 2023 457 0 View

What are the Challenges and Opportunities in Advancing Adult Education in India?

India has made progress in its literacy rate because of adult education programmes but unfortunately adult education in India is seen as a discipline for literacy only. This discussion will...

04 May 2023 6,940 2 View

What is the range for MLVSS in anaerobic reactors?

typically in an anaerobic filtration system or anaerobic reactor treating high strength wastewater

06 April 2023 5,988 1 View

Measurement of Reactive oxygen species (ROS) in Blood using DCFDA dye ?

I want to measure reactive oxygen species in whole blood. Can anyone please suggest a protocol or method for it using 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) dye ?

09 February 2023 3,599 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

How to estimate a protien's size using Chimera or PyMol? how correct this estimated can be?

I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...

21 July 2024 5,732 3 View