I have harvested urine and serum at various time intervals from rats that have been given a crude extract . I want to assess the pharmacokinetics and pharmacodynamics of the individual compounds in the extract. However, I am not sure which method will work better for the pharmacokinetics because I do not know the proportion of each of the compounds in the extract.
Hi,
Unfortunately, there are no such methods. Current solutions and models do not allow for selective analysis of one or several substances after administering the extract (.... which may contain several substances). Such analysis will only inform about interactions between constituents nothing else. Of course, the extent of these PK interactions will depend on the composition of the extract. What you can do is determine the concentration of the dominant substance in the extract and try to determine its concentration in blood samples or urine. However, the cognitive and scientific value of such an analysis will still be low. If an extract is administered, it is not possible to associate a single substance with a pharmacodynamic effect (PD). Currently, we do not have such data analysis capabilities. The effect of PD after administration of the extract is always the result of interactions between all components of the extract. We currently have neither software nor models that can separate these interactions from each other. Therefore, PK and PD are tested with the use of single selected substances or in systems where interactions with one substance are tested, but not with so many.
All you can do is try to characterize the chemical composition of the extract and describe/analyze the PD effect. Selective analysis of PK or selective PD analysis in your case is not possible.
Best regards
Tomasz
So far, a method to selectively evaluate the pharmacokinetics of an extract has not been reported. The extract is a mixture of many chemical components, the proportion of each of them is not known, what can be done is to characterize the chemical composition of the extract, determine the substance that is found in the highest proportion and determine its possible concentration in the sample. urine or blood. At the moment, selectively evaluating pharmacokinetics and pharmacodynamics is not possible, we have neither models nor software that can evaluate them.
Sincerely,
Luis Miguel V. Felix Veliz
Hello Isaac Kyei-Barffour, Please refer following links...
Article Bioactive Markers Based Pharmacokinetic Evaluation of Extrac...
Article Comparative pharmacokinetic analysis of extracts of crude an...
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