medical management of urolithiasis in recurrent stone formation requires estimation of promoters and inhibitors of stone formation in 24 hours urinary and blood evaluation. Can any one give us the best method in collection of 24 hours urinary sample.
It is important to give the patient a suitable container to collect the urine of 24 hours, so a container that can hold at least 2 liters (diuresis may be more important in some patients) and if possible in which he can urinate directly. The patient should start collecting the morning at a definite time. The simplest is when patient rises. He urine as usual in the toilet, but from that moment, he must retain all its urines, absolutely all. If the patient urine once in the toilet, he will have to start all over again! He collects his urine in the container before going to bed and get up at the same time as the previous day. He collects his urine into the container. He has a complete collection of 24H urine: day and night.
thank you Martin
I would like to know whether any reagent need to be added in the container and how many days it is necessary to collect to get correct values
You are asking a question that I do not think anyone really resolved. Indeed, the suggested additive varies depending on the constituent analyzed. The urine stored at room temperature becomes alkaline which may alter the dosage of phosphate and calcium. For the determination of oxalic acid also it is advisable to acidify the urine. But this acidification can be harmful as it will promote the precipitation of uric acid. In addition, acidification may alter some assays performed with enzymes. In practice, one does not use an additive. The urine should ideally be stored in the refrigerator or in a cool place during the 24H of the collection which is not always possible for the patient. So, if possible, the patient after placing the urine of the night in the container shall be to the laboratory as soon as possible so that the urine may be analyzed the same day. Do not exceed 24 hours. Say to the patient to bring all the bottles he had collected. This is the lab that must collect and homogenize them!
24 hour urine should be kept in refrigerator or long preservation and sodium azide is generally used to prevent any bacterial and fungal growth in it
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