Hello, I'm planning to assess insecticide resistance of aphid and its parasitoid along with selection. There are many assays I've found in articles so far.
There are leaf dipping assay, feeding insecticide mixed with 10% sugar solution, bial assay(using residue after drying liquid in a bial).
These are the methods using insecticide directly to adult parasitoid.
And some suggested that using insecticide to the infected aphid(developing parasitoid larvae) is better.
I can't decide which method is more appropriate, but I think that contacting insecticide to adult female only make a selection slower.
Hello,
This depends on the objectives of you study. You have to decide your method depending on the result of the LR, anyway concernnig aphid resistance evauation see the following website and I hope it gives you a practical experience.
https://www.youtube.com/watch?v=jE359LnpBnI&t=228s
Goodluck.
Typically the method involves developing multiple dose-response curves. This process is easier if the residue is applied uniformly because you have then eliminated variability due to toxicant distribution. This is great so long as all of the resistance in the field is due to biochemical mechanisms. This approach minimizes any behavioral sources of resistance.
Selection pressure is up to you, but a few individuals need to survive and be sufficiently healthy to reproduce. You can use the LD10, or LD50 dose as the selection tool.
Match exposure in the lab to exposure in the field. A systemic insecticide might be best tested mixed with diet, while a contact insecticide test might be more relevant as a thin film.
Be aware that "uniform" to you may not be uniform to the insect. The aphid interacts with its environment often at a sub-millimeter scale to distinguish one cell in a leaf from another.
Sometimes these assays are conducted by placing a droplet of pesticide on the insect cuticle using a microapplicator.
In addition to the excellent sugestions of Hanna M. Madanat and Timothy A Ebert, I would advice that you begin and complete your work with easiest system: the aphid. Good luck in your research.
Hi, you can consider using the in vitro aphid assay - Article Establishment of in vitro soybean aphids, Aphis glycines (He...
Leaf dip method is easy. but as you study both aphid and parasitoid try to apply insecticides directly and in invitro condition. After having idea we can focus on more accurate method.
Good luck
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