What is the best medium and method for isolating Cryptococcus neoformans from bird feces?
We have developed Sunflower seed medium ( Pal medium) in 1980 for the rapid isolation and presumptive identification of Cr.neoformans from clinical and environmental sources.This medium is considered the best as per the studies done by some researchers.
We have also did genetic mating of Cr.neoformans on our Pal sunflower seed medium and published paper.
We have published over 45 papers on Cr.neoformans and cryptococcosis in the last 50 years.
You please try our Pal sunflower seed medium in your laboratory, and tell me the results.
If you wish, you can download our papers.
I am attaching my few papers on Cr.neoformans.
Dear Dr.Hawraa,
We are working in the field of Medical and Veterinary Mycology since 1973.
I wish to inform you that colonies of Cr.neoformans imparts light to dark brown pigment on Pal sunflower seed medium that helps in early recognition of this medically important yeast.
Our medium is a less expensive tool for the study of Cr.neoformans.You can also try our simple medium for the isolation of Candida albicans ,Geotrichum candidum, Rhodotorula glutinis etc.
Stay safe and healthy.
With best wishes and good luck,
Prof.Dr.Mahendra Pal
Founder Director of Narayan Consultancy on VPH and Microbiology,India
Possible without crowding to send me a way to prepare the mediumsunflower seed medium
I am attaching my one paper on "Use of Pal’s sunflower seed medium for an early diagnosis of cryptococcosis"
The composition of Pal’s medium is as follows:
Pulverized sunflower seed 45 gm
Agar 20 gm
Distilled water 1000 ml
Chloramphenicol 100 mg
pH 5.6
You can prepare the medium by boiling 45 gm of pulverized sunflower seed with 500 ml of distilled water for about 20 minutes, filter it in two to three layers of muslin cloth,make the volume 1000 ml by adding distilled water, add 20 gm agar powder and autoclave at 110 degree centigrade for 20 minutes, add 100 mg of chloramphenicol after autoclaving ,and pour in the sterilized plates. Incubate one plate at 25 and another at 37 degree centigrade to check the sterility.
You can use this medium in your laboratory for the study of Cr.neofomans and other fungi.
Hi, In my best knowlege, the best medium in your feild is the bird seed agar and\or dextrose bromothymol blue thymine medium, so I mentioned below the links of some papers that may help you;
1. Article Isolation of Cryptococcus neoformans and other opportunistic...
2. https://dergipark.org.tr/tr/download/article-file/128839
3. Article Cryptococcus spp. isolation from excreta of pigeons (Columba...
Kind regards
AbdulKadri
the new culture medium based on a modification of Dichloran Rose-Bengal Chloramphenicol Medium (DRBCm) also used to detect colonies of Cryptococcus neoformans
Recently, an experiment conducted by Katiyar and others (2011) to assess efficacy of five media namely Tobacco agar, Mustard seed agar, Niger seed agar, Sunflower seed agar, and Henna agar conclusively established that sunflower seed medium was the best medium with the mean day of growth and pigment production being 1.25 and 2.8, respectively .
Katiyar R., et al. “Comparison of different media for the pigment production of Cryptococcus neoformans”. Journal of Clinical and Diagnostic Research 5.6 (2011): 1187-1189.
Very recent paper by Pal (2022) mentioned the efficacy of Pal sunflower seed medium for the isolation and identification of Cr.neoformans from avian droppings.There was no isolation of Cr.neoformans on Sabouraud dextrose agar from bird excreta.
If any scientist is working on Cryptococcus neoformans, I advise to use Pal sunflower seed medium as a selective medium , and inform me the results on my following email. Our medium has proved very sensitive, specific and low cost tool for the study of Cr.neformans.
Prof.Dr.Mahendra Pal
Founder Director of Narayan Consultancy on VPH and Microbiology,India
Dear Dr.Hawara,
I am attaching you our one paper in which you can see the brown pigmented colonies of Cryptococcus neoformans on our Pal sunflower seed medium.
The colonies of Candida albicans appear white colored.The brown color effect helps in the early isolation and presumptive identification of Cr.neoformans.
Stay safe and blessed.
With best wishes,
Prof.Dr.Mahendra Pal
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