There are several protocols for real-time PCR for miRNAs. What will be the best in terms of purity of the sample?
I usually use miRCURY™ RNA Isolation Kits for total RNA and miRNA isolation then for first stand synthesis use miScript II RT Kit from Qiagen then for Real time PCR profiling of mature miRNA use miScript SYBR® Green PCR Kit
Purelink miRNA isolation kit of ambion for purification of small RNA.
Superscript III reverse transcriptase of invitrogen for reverse transcription.
SYBR green of Clontech Tamara for real time.
mirVana Isolation kit (Ambion)
If you need higher yield, mirVana PARIS for total RNA isolation is supposed to be better!
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677285/
mirVana kits work great
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