What is the best in-vivo model for hair growth?

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Useful architecture for bioinformatics?

Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...

08 February 2021 2,727 5 View

How to fabricate a thin layer of PEI film without defect?

I want to cast a thin layer of Polyetherimide/DMAc dope solution with thickness of about 50 micron on a glass to fabricate an ultrafiltration membrane via phase inversion method. Can any one give...

14 August 2020 247 3 View

How can we reduce PEI porous membrane shrinkage?

We are producing PEI membrane as support for gas separation membranes via phase inversion method by casting solution of PEI/NMP and water as coagulant. We cast on polyester support but membrane...

14 June 2020 2,324 1 View

Does anybody know the reason?

Hi Everyone I'm working on Targeted drug delivery using Aptamers. I synthesized PLA-PEG-COOH and afterwards I tryed to link Aptamer via linkage of -NH2 in Aptamer and -COOH in PEG. first I...

14 January 2020 9,140 1 View

Why does my PCR, that i optimized, now give weak bands or smears?

08 January 2018 1,985 5 View

How can I get good PCR protocol for the exon4 of LDLR gene?

03 January 2018 1,696 4 View

What is deep trap and shallow trap in semiconductors and how do they affect the conductivity of the alloy involving 2different n type semiconductors?

I mean if the shallow traps and deep traps have different effects in conductivity why we don't investigate them separately ?

04 January 2017 5,755 4 View

Calculate the percentage of mono cations and dications and neutral species in solutions with different Ph ?

Dear all, would you tell me how to calculate the percentage of mono cations and dications and neutral species in solutions with different Ph ranging from Ph=1 to Ph=12.5 ?

28 February 2016 9,426 2 View

How can I solve "unknown function or operator" error in comsol ?

Hello, I used Mathematics>PDE interfaces in comsol. I specified parameters and then I wrote degradation formula in variables part and so on. At the end, when I pressed "compute", I got this...

14 February 2015 7,634 3 View

What are the oxidation potentials of Mo and Nb of 4 and 5 oxidation states in acidic and basic solutions?

Hello there, Can anybody help me to get data regarding redox potentials of Mo4+/Mo5+, Mo3+/Mo4+, Nb3+/Nb5+ and Nb4+/Nb5+ in both acidic and basic solutions? Thank you in advance.

21 January 2015 4,467 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View