There is less information available for human cortex about optimal golgi staining than for mouse or rat. But the tissue I am working with is human and has been in formalin for many years.
I am specifically looking for dendritic spines for classification purposes.
Any protocol ideas or publications for the optimal human cortex golgi staining protocols?
These guys say that past 6 months it gets too inconsistent to try: http://www.neurostructural.org/default.asp?page=80788
But seeing as that is not very helpful these guys posted some papers on it in another thread on research gate. Look at the responses. They may help https://www.researchgate.net/post/Can_anyone_help_with_Golgi_staining_and_spine_analysis
We have been using the Golgi-Kopsch modification with potassium di chromate in paraformaldehyde and silver nitrate for the impregnation for archived human brain tissues.Hope this helps.
- Badges
- Science topic
- Similar topics
- Methodology
- Laboratory
- Histological Techniques