Hailee Rogers

5 Questions 4 Answers 0 Followers

Questions related from Hailee Rogers

What is the best protocol for staining zinc deposits in tissue?

I am trying to stain zinc deposits in human brain tissue but cannot find a decent protocol. I found a couple protocols using dithizone with cell cultures, but I need one for slide-mounted tissue....

09 September 2015 9,846 2 View

Why is my Western blot with human striatum for ceruloplasmin not working?

I am doing western blots on human striatum for ceruloplasmin but cannot get any bands. I know that the concentration of ceruloplasmin in the brain is quite low. So I've tried using a higher...

09 September 2015 9,614 4 View

No ponceau stain despite successful western. What's wrong?

I am doing westerns with human brain tissue and the westerns seem to be working fine. I'm getting a nice band of the right weight, but nothing shows up when I ponceau stain. But here's the thing:...

06 June 2015 255 3 View

Has anyone tried to revive fixed tissue for western blot?

I am specifically working with human brain tissue. Most of the tissue has been in formalin for 2-10 years. I have read a couple papers claiming to have revived their tissue with certain...

05 May 2015 9,842 5 View

What is the best Golgi staining method for human tissue that has been fixed in formalin for many years?

There is less information available for human cortex about optimal golgi staining than for mouse or rat. But the tissue I am working with is human and has been in formalin for many years. I am...

04 April 2015 5,456 2 View