Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Do you have papers on hindlimb myology of birds to share?

Hi all! we are looking for papers (or information) on muscles of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than welcome! Thanks!

22 February 2021 7,186 3 View

Marker Set for 3D Motion Tracking of Upper Arm during Pointing Task?

Does anyone have recommendations of a marker set for 3D motion tracking of the upper arm during a pointing task? Most studies I have come across have focused on reaching. I plan to use Vicon Nexus...

12 January 2021 3,198 1 View

Are there any way to make the comparison between different analysis of reliability possible?

10 December 2020 7,922 4 View

How do I calculate variance explained by each predictor in a multiple regression via SPSS?

I have 4 independent variables within a multiple regression model. I am trying to figure out the percentage of variance each individual independent variable contributes to the model using SPSS....

28 November 2020 583 3 View

Does anyone has suggestions to extract carbohydrates from E. coli?

Dear colleagues, We are interested in extracting carbohydrates fro E. coli and the protocol we've seen so far include too many hours in a sonicator. Does anyone has suggestions or experinece in...

26 November 2020 5,907 3 View

Is the statistical analysis performed correct?

Dear colleagues, I have some doubts about the correctness of the following analysis. A class of 79 students underwent three different teaching methods that chronologically presented themselves...

02 November 2020 4,030 4 View

Is there a validated english questionnaire to assess Achievement Motivation in Sport (Hope for Success and Fear of Failure)?

I'm looking for an instrument in english that does not measure goal orientations.

04 October 2020 3,255 5 View

Does anyone know a reliable human ApoB100 ELISA?

Dear research community, I'm looking for a reliable human ApoB100 ELISA to measure ApoB in VLDL particles which were isolated by density gradient ultracentrifugation. Are there any...

28 September 2020 2,841 2 View

How to calculate a-priori sample size for a repeated measures 2x2 ANCOVA?

I am planning an experiment 2x2 within-subjects factors and additionally I would like to consider a covariate, so that a 2x2 repeated measures ANCOVA is the intended analysis. Does anyone know how...

16 August 2020 985 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

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01 March 2021 3,905 5 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View