What is the best expression vector?

Mohammed Al-Nussairawi @Mohammed-Al-Nussairawi

10 June 2019 1 8K Report

Hi,

What is the best expression vector for expressing a protein coded gene into E.coli BL 21 (DE3) plyse chemical cells ? I know that pET vectors are the best choice but which one is good ? for my work ?

thanks in advance

Mohammed

Bccm Genecorner

You should choose an expression vector with another origin of replication than p15A since the pLysE plasmid already carries the p15A origin of replication. The pET3 and pET11 plasmids are available at the BCCM/GeneCorner Plasmid Collection (see http://bccm.belspo.be/catalogues/lmbp-plasmids-search-results?LIST1=NM&TEXT1=pET&search=+++Run+Query+++).

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