T-test and Mann-Whitney U test on groups with different sample sizes and variances?

Hello I have 2 groups with different sample sizes of (experts: 5 to 8) and (Users: 28 to 35) answering 21 questions on a 7 points Likert scale about the importance of some dimensions for...

31 January 2021 3,885 7 View

What are the types of optimization problems that can't be tackled by meta-heuristic and heuristic algorithms?

Any decision-making problem when precisely formulated within the framework of mathematics is posed as an optimization problem. There are so many ways, in fact, I think infinitely many ways one can...

06 September 2020 9,415 14 View

Is it possible to reduce tensile strength and increase compressive strength by adding any admixture to concrete?

Hello, I want to know is it possible that the compressive strength of concrete increase by reducing tensile strength? if yes, how we can explanation and justification this issue?

30 April 2020 4,489 7 View

What are the plans for exams and assessments of universities during corona virus pandemy?

What alternative ways universities use for academic assesment of students?

29 April 2020 3,091 1 View

Is there a specific way to discretize a continuous model to any arbitrary multi DOF system and extract the mode shape matrix in abaqus?

27 April 2020 239 3 View

What is best sampling method for cross sectional studies?

I want to research on liver disease patients behaviours and habits in shiraz city. How can I calculate sample size accurately? Thanks

23 March 2020 9,974 5 View

Anyone in kolkata working of LNCaP prostate cells?

I am working with prostate cancer cells, i want to know who is presently working with PCa cell lines in viro and in vivo in kolkata.any leeds will of great help.

07 March 2020 7,831 1 View

How can I find the IDT point of the following TGA curve which is related to a clay soil?

The attached file shows the TGA curve of a clay soil. Can anyone help me how to recognize the initial decomposition temperature (IDT) from this curve?

06 February 2020 6,944 3 View

How to isolate dorsal root ganglia in rat at lumber region?

For pathological investigation of sensory and motor nerves in lumber region of rat how to isolate and preserve

04 November 2019 8,974 1 View

What's the procedure for validation test of a new design of product (Say Heat Exchanger)?

after 3D design of all components of the Heat Exchanger geometry we do assembly and finalization of the geometry, in CFD analysis we change the boundary conditions and it refers us to the...

27 October 2019 9,095 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View