Hello Carlos,

I am currently working with C57BL6 mice and I am using between 1.5% and 2.5% DSS. However, the effect of DSS is highly variable between manufacturers and even batches. It would be advisable to determine the optimal concentration to induce colitis in your particular mice strain every time a new batch is used. Attention to mice gender, age and weight should be also paid in order to obtain consistent results.

Regards

Hi Sonia,

Thank you so much for your valuable information. We will give special attention to your recommendations when planning and conducting our experiments.

Best regards.

Carlos