What is the best dosage of tetracylcline in vitro effect?

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What's the better material instead of Glutamine for CHO cells in cell culture?

I've designed media for CHO cells, without Glutamine! But when they're in large scale, the cells were damaged and decreased their viability. What's your refer ?

14 January 2024 2,503 2 View

How can I find PCR markers and suitable antibody to flow cytometry of C2C12 cell line?

How can I find "PCR markers" and "suitable antibody to Flow cytometry" of C2C12 cell line? Is there any web server to find this data?

02 December 2023 3,248 0 View

Can someone provide me with the earthquake repositioning code with Python?

29 September 2023 6,469 3 View

Would using a high ROX master mix cause any issue for PCR instruments that do not require ROX as normalizer?

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22 July 2023 964 6 View

How long can transient plasmids (like PEGFP N1) remain after transfection into bone marrow stem cells?

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06 June 2023 9,768 1 View

Can I import bipolar electrode montage into eeglab?

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12 July 2022 3,546 0 View

Can we extract intracellular cellulose from bacteria which exist in paper mill activated sludge?How?

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02 July 2022 6,182 2 View

Is there any fully automatic MR prostate segmentation?

Is there any free available tool or even demo that gets the MRI image and creates the contouring of different organs? I need to just test some few cases. Thanks.

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How to mature "Oligodendrocyte precursor cells (OPCs)" CELL-LINE to "Myelin producing Oligodendrocytes"?

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With the NCBI Probe Database being retired, is there any similar database I can refer to for finding tetra nucloetide microsatellite markers?

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Geotechnical Engineering (Proceedings of the ICE) time review?

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What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

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Weak DAPI staining after immunohistochemistry - how to improve?

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For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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Why results of ROS flurescence are negative as there was no bacteria within?

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How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

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Saurabh Mandal

In in-vitro conditions, the Drug concentration, or IC50 value mainly depends on cell type and incubation period. With your limited information, it will be difficult for members to provide any suggestions. you can check the papers.

Negar Nekahi

Thank you for your answer. i need it for my final project, its about wound healing. the skin cells are so different, kondrocyt, fibroblast or ... , and i did not choose it. and for incubation period i have not make desicion yet. if you have any information can help it would be helpfull please share it or if you have any helpfull paper it will be my pleasure to read...

Thank you so much

Hi Negar Nekahi

Check this paper.

Article Cytotoxicity Induced by Tetracyclines via Protein Photooxidation

Regards

Saurabh