The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

Is there any general protocol for immunohistology staining?

I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?

17 February 2021 3,062 4 View

How can i figure out whether nanoparticles in my synthesis crosslinked or not?

i prepare protein nanoparticles. in one of my synthesis i use trifle amount of crosslinker and the nanoparticles stick in the bottom of plate. how can i understand that nanoparticles become...

03 December 2020 559 8 View

How to examine the substitution of ligands bound to protein during the MD simulation?

I have simulated a complex ( A protein docked with two ligands), and the ligands are bind to two different protein sites. Still, from the start of the simulation, ligands are positioned in other...

27 November 2020 7,848 4 View

How to change the atom numbers of a residue in a pdb file?

I am trying to dock 2 proteins in HADDOCK server, but it repeatedly shows the following Error for the second pdb file: " Error in PDB file. Your PDB contains multiple residues with number 1 in...

25 November 2020 4,753 1 View

Is it possible that formazan Crystal formation took more than four hours ?

Hello dear scientists: When I treated the Pc12 cell line with H2O2, after 2h the cells seemed to be dead. I didn't observe crystals, so I didn't add DMSO and left it with mtt in the...

20 October 2020 1,106 3 View

How can i detecte the fluorescence and non-fluorescence complex?

hello kind regards I study the structure and activity of the digestive enzymes. The enzyme I studied contains 5 amino acids tryptophan. In one of the studies, I study the effects of a food dye on...

04 October 2020 8,563 3 View

How to increase the number of nanoparticles in fe-sem?

I produce a protein nanoparticle complex,but in fe-sem, I see a few number of nanoparticles,maybe 2 or 3 ,however the color of suspension solution is quite milky and this is a sign of...

29 August 2020 8,812 5 View

Why would a negative control have apoptosis in the Annexin V-PI Flow Cytometry?

06 August 2020 3,627 22 View

How can we induce gastroesophageal reflux disease in rats?

Gastroesophageal reflux disease (GERD) occurs when stomach acid frequently flows back into the tube connecting your mouth and stomach (esophagus). This backwash (acid reflux) can irritate the...

14 June 2020 145 8 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View