Does anyone have experience in DAPI staining in Aspergillus? and how to prepare to get best image using confocal microscopy?
Thank you
I want how their chieftaincy is done and what brought the conflict
01 June 2024 5,315 0 View
Halo, I have a few questions. What if the Multivariate Kurtosis has a p-value > 0.5 (not significant), while the Multivariate Skewness has a p-value < 0.5 (significant)? If the results are...
16 April 2024 2,629 1 View
I am trying to conduct a gummy stem blight melon line screening. However, I had a little trouble initiating spores using the artificial medium. Some papers mention cucumber fruit could become an...
05 November 2023 7,061 0 View
I have read a research paper Ageratum Yellow Vein Virus isolated from a tomato plant with leaf curl on Ishigaki Island, Okinawa, Japan. According to this research, the paper confirmed that AYVV is...
05 November 2023 3,539 0 View
Good afternoon. I want to introduce my self, I am Dede Kosasih from Sundanese Language Education Study Program, FPBS UPI Bandung. I apologize for permission to submit questions and suggestions,...
02 April 2023 1,416 3 View
I just try with EPA PMF 5.0 to determine the source of PAHs in sediment. My data is only onetime sampling and not as monitoring data. Therefore, when I make a dataset, I can not input the date on...
01 November 2021 4,749 0 View
I am trying to validate acrylamide analysis in LC-MS/MS but I can see arylamide at first and second injections after then I could not detect any peak. I am using C18 column and 99.5:0.5...
02 June 2018 2,402 3 View
hello, i have a problem with "nett effects" on regression analysis. why the "net effects" serve to estimate the contribution of each regressor into the model? and how to interpret them? thankyou
22 June 2016 1,599 3 View
What is the genetic influence of necrosis in diabetic ulcers?
20 January 2016 210 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...
31 July 2024 3,913 6 View