I need a complete protocol to see what is the best condition for keeping mice during my experiment especially when I want to make incisions on them.
All the details about mice and housing are provided in the method section of my papers
The animals should be housed in polypropylene cages, maintained under standard conditions of 12/12 hours of light/dark at temp. 25C/ 3C and 35 to 60% humidity.
In the U.S., the American Association for Laboratory Animal Science (AALAS) is the organization that provides guidelines for the institutional animal care and use committees (IACUCs) at research foundations/universities, i.e. explicit guidelines or standards for housing and care of laboratory animals, including mice. Here is a link for U.S. IACUC resources:
https://www.aalas.org/iacuc#.VHSMSIvF8QF
Here's their international reference link:
https://www.aalas.org/iacuc/laws-policies--guidelines/international-government#.VHSMsovF8QE
And here's a link to their course on the care, housing and use of laboratory mice:
https://www.aalaslearninglibrary.org/Pages/Courses/Lessons.aspx?intCourseID=260&intTrackID=0
The guidelines that Dr. Mishra provided above are generally a good idea, and I would add: access to a standardized formula rodent chow, pelleted, and purified drinking water in glass or polypropylene bottles or alternative delivery system (i.e. rack-mounted), ad lib; cages should contain hardwood bedding, and no more than 5 same-sex adults per standard mouse cage; cages should be changed (and washed), and fresh bedding added at least once weekly (twice weekly may be desirable in some situations), and all animals monitored at least once weekly for general health and to prevent morbidity, regardless of study status (i.e. current assignment to treatment/control/other study arm). Monitoring of mouse colony for the presence/control of murine diseases should be done under the direction of a qualified veterinarian, including the use of sentinal cages, as appropriate. There are many other factors that may become much more important to consider depending on the goals of your particular study (i.e. presence of xenobiotics/metals in the bedding, phytoestrogen or other "trace" contents of the mouse chow, identity of metals/organisms in the drinking water, etc).
Here's a good reference on what kind of study design/animal housing information should be reported in publications:
http://www.ncbi.nlm.nih.gov/pubmed/24481642
I hope you find the answers you seek!
I suppose you mean a skin incision. For a start you have to evaluate, not just the post operative pain of the incision, which could be pre-emptively treated with lidocain -skin infiltriation , but also about what kind of stimulus/pain, the material (biogenic or else) to be inserted s.c. might cause over time. Mice feel at best at around 26°C, but (for open thorax surgery , 28-30°C should be the ideal recovery temperature, in addition with glucose enriched drinking water and an adequate analgesic regime Aslo bear in ming the larger the skin shaving area will be, the more body heat will be lost during surgery and recovery. It's always helpful to provide mice with plenty of nest bildung material, a kind of shelter, autoklaved tissue paper or special shredded paper.