How we can grow the HT-29 cells on transwell for TEER? Is there any specific media we have to use??

Is there anything that we have to add in media for the differentiation of HT-29 cells or HT-29 MTX specific cell line or any differentiation inducer is used for TEER estimation in HT-29 cells??...

21 November 2020 7,243 1 View

Is this pandemic an outcome of globalization?

The societies around the world are fighting a battle to combat the further spread of Covid-19. This pandemic can be understood as a direct consequence of extensive globalization. Will this...

05 October 2020 258 3 View

Pls give suggestions about journal where article related to biomass( TGA ftir proximate analysis of seed) can get published soon?

Article includes characterization like TGA, FTIR and proximate analysis of 3 different types of seeds?

19 September 2020 3,455 3 View

Can anyone suggest which primer(s) (oligo dt/random hexamer or both) would be good to generate E. coli cDNA?

Knowing that very low percentage of E. coli mRNA is polyadenylated, perhaps random hexamer should also be used to generate even the 1st strand of cDNA, instead of oligo (dt) primer?

29 August 2020 6,515 3 View

Can i reuse Ni-NTA column for the same protein? Also, washing with 0.5 M NaOH didn't seem to work (as per the manual))?

Can i simply wash with MilliQ to wash off the imidazole from the column in order to run the second set of purification of the same protein??

14 December 2019 5,541 10 View

What are the paid scopus indexed journals in physics?

looking for a paid and fast (in terms of publishing) scopus indexed journal in physics or material science

17 June 2019 983 3 View

Have you used repertory grid interviews?

What have you used these for and where to learn about the various analysis that can be conducted using this method?

23 March 2019 8,369 2 View

Regarding NATIVE PAGE streaky pattern?

I purified my lectin from a seed by ion exchange chromatography followed by gel filtration chromatography.The eluted fractions after gel filtration chromatography were used to perform a...

23 August 2018 2,151 2 View

Is there any short term(0-3 months)course which can help with understanding Research Methodology & Data Analysis in depth?

This can be anywhere worldwide. It can be a certificate course or a summer school programme-but it must be on campus. If it concentrates especially on social sciences research or even management,...

20 March 2018 5,448 8 View

Hello everyone

19 May 2017 9,043 3 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View