What is the best B1 agonist available?

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How to identify wetland area in Landsat imagery?

The indexes NDWI and MNDWI are use to identify waterbody. Can I use these index to identify wetland also? Thank you for advance.

25 July 2024 6,242 2 View

Im trying iodination of an arene, but only getting black precipitates, is there anyone with iodination experience who can advise?

Organic Synthesis. My student has been trying iodination of arenes (our goal is to iodinate p-phenylenediamine) following these literature procedures: 1. Synthesis 2004, No. 11, 1869-1873 using...

16 July 2024 6,630 1 View

Is there a way to calculate cell density and thus cell count from confluency for RPE-1 cells?

I am growing cells in a 24 well plate. I do not have access to a microscope with a camara, so I have to calculate the number of cells in the wells manually. I can get the confluency pretty easily,...

04 July 2024 9,622 0 View

Why am I seeing no color change of the positive control cell death assays?

I am currently running Cell Death ELISAs, using the Roche Cell Death Detection ELISA kit. My samples are from the supernatant of bacterially infected primary canine cells. I am seeing no color...

02 June 2024 8,682 1 View

Latest Advancements in Minimally Invasive Heart Surgery ?

What are the latest advancements in minimally invasive heart surgery techniques, and how do they compare in terms of patient outcomes and recovery times to traditional open-heart surgery?

16 May 2024 3,336 2 View

What would be some simple definitions to give to coaches and athletes on force platform metrics?

Jump Momentum Force at min Displacement Avg Propulsive Force Braking RFD Avg Braking Force

12 May 2024 768 0 View

¿Como puedo transformar una variable donde predominan los ceros y también hay valores altos dispersos?

Tengo una columna de la variable de "precipitación" donde predominan los ceros y hay valores altos. No he podido transformarla para obtener una distribución normal, ya he usado métodos de log,...

05 April 2024 8,427 1 View

Is it necessary to include an FMO for 7-AAD dye in your Flow Cytometry experiment? If not, why?

I'm optimizing a flow cytometer experiment and one of my three stains is a 7-AAD Live/Dead stain. The other stains in my experiment are staining for CD34+ and CD45+ cells. Is it necessary to...

01 April 2024 3,060 0 View

Would you have a simple Protocol for Flow cytometry cross match test (in flow tubes)?

The cross-match test is an in vitro test to determine the presence of anti-lymphocyte antibody to donor cell antigens (lymphocytotoxic antibody) in serum of an individual with preformed...

01 April 2024 3,203 1 View

Has anyone observed an injury caused by a spiny dogfish sting resulting in isolated muscle atrophy in human?

Fisherman stung while extracting hook from fish.

19 March 2024 535 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Can we eliminate the stress singularity at the tip of the crack by manipulating the elastic constants?

The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.

25 July 2024 9,976 3 View

Can you visualize platelets using EVOS ?

Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...

24 July 2024 9,337 3 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

Deepak Atri

I think it would depend on the experiment and the particular parameter you are trying to measure. Consider isoprenaline or xamoterol. Also, if you are trying to do something clinically-relevant, the parasympatholytic atropine might also be worthwhile.

Paul Ernsberger

Administer a nonselective agonist along with a beta2 antagonist.

Ahmet Adiyaman

We use isoprenaline for bradyarrhythmias and dobutamine as inotropic. You could consider a higher dose of dobutamine or adding noradrenaline to dobutamine.