i am preparing a nanoparticles based drug delivery system to 5-Fluorouracil.
i want to evaluate how such drug delivery system could affect or enhance both the pharmacokinetics as well as pharmacodynamics of 5-Fluorouracil??
what is the best cellular target should i analyse to determine whether this drug delivery system is enhancing or not??
can thymidylate synthase or other target enzymes in the downstream pathway of 5-FU help in this??
i appreciate any contribution
Is DNA or RNA abnormalities resulting from incorporation of false pyrimidines represent an appropriate readout??
The idea is to construct a new dds beased on nanomaterial. I would not care so much about molecular targets, I would rather focus on the pharmacokinetics and in vivo studies on anticancer activity. In the case of PK studies on oral formulation it is important to remove the whole GI tract for the determination of the %D remaining there.
Thanks so much Dr.Bale,
i will do cell cycle analysis by flowcytometry for the invitro experiments after few hours of exposure. Also invivo pharmacokinetics will be done.
Best Regards
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