What is the best approach to extraction of onions bulbs for antimicrobial studies?

More Alloysius Chibuike Ogodo's questions See All

I need sample of APA 7th referencing?

APA 7th referencing style in details

02 October 2023 9,619 1 View

Can anyone help me with MATLAB code to implement (1) Double sliding window method of packet detection in OFDM (2)Delay and correlate method ?

I am working on developing a robust receiver for an 802.11a wireless receiver, Can anyone help me with MATLAB code to implement (1) Double sliding window method of packet detection in OFDM...

30 August 2019 7,075 3 View

How do you calculate colony forming unit per millilitre (CFU/mL) of a bacteria cell using Spectrophotometer?

CFU/mL of sample can easily be determined using solid medium. However, difficulty is always encountered using broth culture in spectrophotometer which may be considered more appropriate by some...

04 November 2018 7,037 11 View

How can one estimate the concentration of an aqueous extract of plant material in mg/mL?

The method of estimating the concentration of the extract in mg/mL. Formular and process is required.

07 February 2018 3,045 11 View

I need a complete protocol to study gene expression in yeasts using real time PCR (q-PCR and RT-PCR) and Microarrays. Can anyone help?

A complete protocol for the study is required

02 November 2017 4,469 6 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

Esther Del Florence NDEDI Moni

Hydrodistillation using a Clevenger apparatus to obtain onion essential oil

M. C. Sidhu

I think the use of fresh onion bulbs is the best material to extract maximum number antimicrobial compounds for study. On drying, onion bulbs may loose some of the volatile compounds which are likely be antimicrobial in nature.

Alloysius Chibuike Ogodo

Dear Esther Del Florence,

Thank you for your contribution.