The method of estimating the concentration of the extract in mg/mL. Formular and process is required.
Dear Dr. Alloysius,
Thanks for your question. Please visit: https://www.researchgate.net/post/I_have_crude_extract_from_medicinal_plant_the_yield_20_how_can_make_stock_solution_and_then_serial_dilution_500_250_125_626_and_325mg_ml
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Egbuna, C.
One method consists in carrying to dryness a measured aliquot of extract, eg. 10 ml, (up to constant weight) and then weigh the amount of solid residual.
A calibrated container is needed in which to insert an exactly measured extract volume.
The evaporation methods that can be used may vary depending on the equipment available and the type of solvent to be removed.
Dear Dr. Alloysius,
Thanks for your question. Please visit: https://www.researchgate.net/post/I_have_crude_extract_from_medicinal_plant_the_yield_20_how_can_make_stock_solution_and_then_serial_dilution_500_250_125_626_and_325mg_ml
Should you need further guide, please visit our project of more than 150 experts to help you out: https://www.researchgate.net/project/Collaborative-Book-Project-on-Phytochemistry
Thank you.
Kind regards,
Egbuna, C.
Dear Dr. Alessandro Venditti,
Your answer is highly appreciated
Thank you Dr Egbuna, your references were very useful. However, the second link did not provide specific chapter for the question.
Dear Dr. Alloysius,
Thank you so much. I am glad that the references were useful. Regarding our project link, the first volume is under evaluation by the publisher. I am sure it will be very useful because the various methods including reagent preparation and state-of-the-art methods were presented.
Although we are no longer calling for contributors but you may wish to join us for subsequent projects and for possible collaboration that is capable of taking your research activities in the area of phytochemistry to the next level.
Thank you.
Kind regards,
Egbuna, C.
Dear Dr. Dickson,
Thank you so much for your kind remarks.
Regards,
Egbuna, C.
You can estimate the concentration of the plant extract by the following steps:
1. Bring a small flask or jar
2. weight of the jar or flask (assume that weight 100 g)
3. Take a specific size (10 ml)
4. Place it in the jar or the flask
5. evaporate the solvent completely
6. weight of the jar or flask (assume that the weight 100.1 g)
7. Dry extract weight =100.1-100 = 0.1 g
8. Concentration of plant extract = 0.1g /10ml = 100mg/10ml = 10mg /ml = 10000 µg / ml = 10000 ppm
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