Dear my friend

At first, I would like to greet you on your subject

According to my humble experience, generally the concentration used in this test ranged between 2 to 3 mL/kg b.w (1:1 mixture of CCl4 and olive oil)

In such tests is taking into consideration the injection place/position

I wish you all success

Best regards

Abderrahim

According to my knowledge, 0.4 mL/100 g body weight with ccl4: olive oil (1:1) is considered enough for acute hepatic injury.

Hi,

We find in our experimentation 1 ml /kg b.w (1:1 mixture of CCl4 and olive/peanut oil) i.p. is appropriate to induce hepatic injury in adult mice.

Best..

Hi,

@ Amirouche Deghima which concentration did you try and what was the outcome?

@ Umesh Tharehalli , So sorry for my late answer !!

i worked previously with ccl4 0,1 % and 10 ml / kg b.w and there was no hepatic injury observed ??