I am doing solid state fermentation by using commercially prepared media e.g PDA, SDA. It is really hectic and time consuming to prepare and inoculate hundreds of plates. Is there any alternative?
The best strategy before mass cultivatiosn is to find out the condition in which the endophyte will produce the maximum amount of secondary metabolites. Many fungi maximize their MS production under stress; it is frequently osmotic, but it also may be stress induced by low nutrient concentration. Other fungi maximize production under high nutrient concentration at the beguinning of colonization; this is particularly true with early colonizers where fungal MS are supposed to exclude competitors from secured food. Another factor to determine is the culture age when the MS should be harvested
Once you know the conditions to MS maximization you can mass-culture your endophyte on autoclaved leaves of the host plant (it may be enriched) or do as Dr. Banerjee suggests.
You can prepare Potato dextrose broth, add them into few flasks and inoculate the flasks with the fungi and allow the fungi to grow in the medium while in a shaker or still. And after leaving it for the required time period (first you have to find out at which stage, i.e. on which day in the growth cycle, the fungi start producing the secondary metabolites) you can extract the fungal metabolites from the medium into methanol or ethyl acetate. Growing the fungi in liquid cultures give more crude than in solid medium and it avoids time which takes to inoculate many solid medium plates.
Yes of course you need a lot of inoculum. For SSF of Aspergillus niger I used dry inoculum collected from thin SSF of the mold on steamed rice. The product was dried with blower oven. For Eupenicillium javanicum I used liquid culture.
HI All, I urgently need method for solvent extraction of secondary metabolite(lignan) from fungal cultrure.Can anyone help
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