What is the best alternative for MOPS buffer in RPMI1640 Medium in order to do antifungal activity of natural products test?

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Greg Cote

In terms of pKa only, sodium or potassium phosphate can be used to give buffers in the same pH range as MOPS, around 7.2. However, I don't know if the presence of extra phosphate will affect your yeast growth. Other buffers with a similar pKa are BES (pKa 7.15) or TES (pKa 7.5).

Román Y Ramírez-Rueda

Thanks a lot Greg.