Try Sabouraud agar

Dear Razi

try water agar only

Best regard

Dear

I recommend PDA (Potato Dextrose Agar)

If you wish to suppress size of mycelial fungal colonies, try Rose Bengal agar - https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/RoseBengalAgarwChloram.htm

Hi Razi

You use PDA better

Dear , Razi Zulkepli.

You can use PDA , but you must make series of dilutions and apply the last dilution for example 10-4 in order to avoid the interactions among fungi colonies. 

You can see:

http://www.microbiol.org/resources/monographswhite-papers/counting-colonies/

You can also see the attached items ..

Best regards

Hi: Water Agar is perfect to this mission. 

try Sabourad agar 

Sabouraud agar

Dear Razi

Try DRBC Agar, as it possesses bengal rose and dichloran inhibits excessive or exacerbated growth of filamentous fungi allowing you to make your UFC count. You can also try with DG18 agar, although I recommend the former for safety.

Best regards

Dichloran Rose Bengal Chloramphenicol agar is the best choice for counting of the moulds

Potato dextrose agar with Chloramphenicol works out to be good

Dear Zulkepli,

Potato dextrose agar with Chloramphenicol, Rose bengal Chloramphenicol agar, V8 Juice agar, Malt Extract agar works well to isolate and differentiate fungal colonies.

also Sabouraud agar is good to isolate  fungal colonies

Depends on the fungus, but you could use Sabouraud agar with some antibiotic

Potato Dextrose Agar is the best.

Conidia of what fungus?

I agree with those that suggest Sabouraud agar with some antibiotics such as Penn/Strep and/or hloramphenicol.

Don ;t use the antibiotics unless you are working with potentially contaminated preps. 

Hi everyone, thanks for all the suggestions.

I spent some time experimenting with most of suggestions with many input from literature and finally found the best that worked for me (ymmv though). I was counting the CFU from conidia of Trichoderma sp from potentially contaminated source.

Using PDA as base, for every 1 liter of PDA, add the following:

-150mg of rose bengal

-250 mg of chloramphenicol

-50mg of streptomycin

-1ml of Triton-x 100

-optionally 150mg of PCNB for extra assurance against large  chunk of potential fungal contaminant.

PDA base allows for fast growth of colony.

Chloramphenicol and streptomycin eliminates bacterial contamination.

Rose bengal and triton-x acts as  retardant that keep the fast-growing colony of trichoderma to grows in form of small, compact and highly visible discrete  patch As added extra, rose bengal is also antibacterial and adds mild contrasts that helps improve colony visibility.

After several tries, I skipped PCNB there are almost no significant fungal contamination on my samples.

Ill try to post the picture later.

One caution - control the light exposure for Rose Bengal containing media.

http://www.jstor.org/stable/3626929?seq=1#page_scan_tab_contents

In general PDA is good but I think media selection depends of fungal species too

Regards

Try to use Rose Bengal agar.....

141

Hi Razi

You can use the water ager

84

You can read some useful references in attachment 1

https://www.sciencedirect.com/science/article/pii/S0944501305000133

2

http://www.sas.upenn.edu/LabManuals/biol275/Table_of_Contents_files/14-Enumeration.pdf

3

https://www.microbiol.org/resources/monographswhite-papers/counting-colonies/

4

https://www.sciencedirect.com/science/article/pii/S0944501305000133

5

http://www.sas.upenn.edu/LabManuals/biol275/Table_of_Contents_files/14-Enumeration.pdf

6

https://www.jove.com/science-education/10030/filamentous-fungi

7

http://www.moldbacteriaconsulting.com/fungi/colony-forming-units-cfu.html

8

http://www.moldbacteriaconsulting.com/fungi/fungal-spore-trapping-and-enumeration.html

9

https://www.synbiosis.com/assets/docs/Technical-articles/DWS-017-12-Synoptics.pdf

10

https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/RoseBengalAgarwChloram.htm

Good Luck