You should specify what's your target cell types. If you want to infect the maximum number of total neurons and astrocytes, I recommend you to use AAV9

injection in ventricle. But a tail vein injection of AAV-PHP.eB would give you much more uniform brain infection.

You could find more information about AAV production and application in this website: https://www.genemedi.net/i/aav-packaging

It will likely depend on which cell types you want to target.

See, for example: Hammond SL et al. Cellular selectivity of AAV serotypes for gene delivery in neurons and astrocytes by neonatal intracerebroventricular injection. PLoS One. 2017 Dec 15;12(12):e0188830.

I agree that you need to specify which cell types you'd like to target. That will determine the best serotype. I've used both AAV5 and AAV9 for gene therapy for protein integration.

You should specify what's your target cell types. If you want to infect the maximum number of total neurons and astrocytes, I recommend you to use AAV9

injection in ventricle. But a tail vein injection of AAV-PHP.eB would give you much more uniform brain infection.

You could find more information about AAV production and application in this website: https://www.genemedi.net/i/aav-packaging

1、Intraventricular injection of adult mice can be performed with AAV9 for stereoscopic in situ injection to infect the brain area you need to infect; or AAV-BBB, a serotype that can cross the blood-brain barrier, infects the whole brain via tail vein injection.

2、Infecting the ventricles of mice, the heart can be infected to the maximum extent by in situ injection of the heart, without infecting other tissues.