I have bacterial strains, and I want to do 16sRNA sequencing , which option is best (economical and quality ) considering that I am located in Egypt ?
If the strains are in pure form, just use the above mentioned primers. This will be convenient.
Run a colony PCR with the above mentioned primers. Irrespective of any place, it gives the best of the results.
If u r asking about the 16S rRNA gene sequencing service providers, it is the same as any DNA sequencing service. Just ask the sequencing service to use the primers you have used during PCR amplification of the gene.
If you are asking about the service for 16S rDNA identification of bacterial strains, i.e., the whole service, you can contact DSMZ in Germany.
Alternatevely, you can collaborate with any group doing this in ur Uni or any in Egypt.
All the best wishes
DSMZ is:
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures
http://www.dsmz.de
Dr Yaseer, You can inquire to us. Xcelris provides 16S rRNA Sequencing service on illumina MiSeq Platform (Paired End 300 bp). The V3-V4 (Product size ~459bp) region of 16s ribosomal RNA gene from bacterial metagenomic DNA will be amplified using specific V3-F and V4-R primers with overhang adapter via PCR and sequenced on NGS - High throughput & read length provider sequencing platform. If you wish to cover the other region (V1, V2,..V9), We can cover accordingly. Please feel free to contact me on [email protected] / Skype: devarshi.xcelris
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