This questions aims to enquire the most appropriate way to prepare the starter culture for Pilot scale E.coli fermentation at industrial level. The biggest concern being protection against loss of clone and batch to batch variations.
You can first start from frozen stock and grow the E. coli in 5mL LB media for 8-12 hours, take OD, and transfer to a 1L culture or higher for a preculture. After letting that grow for another 8-12 hours you can then transfer to a fermenter, depends on the size.
You could try this recipe out: https://patents.google.com/patent/CN111073836A/en
Best of luck
Thank you Raghavan Sivaraman for your suggestion. I will look into it surely.
Kind regards!
Start by inoculating a small volume (e.g., 50-100 mL) of the selected medium with a small amount of your stock culture. Calculate the volume of the pre-culture needed to inoculate your 3-5L fermentation batch. The inoculum size should be sufficient to achieve the desired starting cell density in your larger culture, typically specified as an OD or cell count per mL.
- Badges
- Science topic