I am isolating EDL muscles in c57 mice and am subjecting them to stretch and electrical stimulation to measure specific tension, PSCA, total force, and force recovery.
Krebs-Henseleit Ringer’s solution: 117 NaCl, 4.7 KCl, 2.5 CaCl2, 1.2 MgSO4, 24.8 NaHCO3, 1.2 KH2PO4 and 11.1 glucose; concentrations in mmol l-1 (Burton, 1975)
I have retrieved a study that reports a logistic regression, the OR for the dichotomous outcome is 1.4 for the continuous variable ln(troponin) . This means that the Odds increase 0.4 every...
14 November 2023 8,513 3 View
I have the OR of a logistic regresion that used the independent variable as continuous. I also have the ORs of 2x2 tables that dichotomized the variable (high if >0.1, low if < 0.1). Is...
13 November 2023 8,230 4 View
In my 3D printing of zirconia research, I have found a common trend to be an increase of transmittance (%) with increasing wavelength for my sintered samples. I have attached one example plot of...
25 January 2022 4,391 1 View
Is there a best method for simulating a tensile test with a strain rate of 1mm/min that experimentally took minutes while obtaining accurate break results? Is it necessary to run at a specific...
03 August 2021 3,221 2 View
Hi all, I’ll paste the more specific version of the question below before unpacking what I’m asking: In psychometrics, what are some established quantitative methods to examine competing...
13 January 2021 2,937 3 View
Hi there, Please see the attached table for context. Please note that each year they were sampling new freshmen (i.e., not within-subjects longitudinal design). So, the authors of a book I read...
14 October 2020 6,698 3 View
For background: We have a polymer and are looking to adjust its thermal and mechanical properties to resemble the properties of a commercially available polymer to present it as a viable...
17 August 2020 2,580 5 View
Hello! I ran a logistic regression with a categorical IV (control, low experimental, high experimental) and a dichotomous DV (no/yes). RQ: Were participants in the low or high experimental...
01 February 2020 454 2 View
I am looking to simulate the injection molding process for tensile specimens of a novel PHA. The simulation requires elastic modulus and shear modulus as parameters which I don't believe can be...
24 June 2019 2,235 2 View
Hi, I'd like to increase the hydrophobicity of a glass substrate (or lower the surface energy) without adding any coatings / layers. What are some common methods to achieve this?
27 January 2019 708 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View