In psychometrics, what are some quant. methods to examine several competing operationalizations of the same construct to see which is most 'valid'?

13 January 2021 2,740 3 View

How can percentage change over time be assessed/analyzed with multiple time points without the sample size (or any other info.) at each time point?

Hi there, Please see the attached table for context. Please note that each year they were sampling new freshmen (i.e., not within-subjects longitudinal design). So, the authors of a book I read...

14 October 2020 6,530 3 View

What is the best way to determine the optimal polymer additive blend?

For background: We have a polymer and are looking to adjust its thermal and mechanical properties to resemble the properties of a commercially available polymer to present it as a viable...

17 August 2020 2,427 5 View

Interpreting confusing SPSS logistic regression output with categorical IV (3 levels) and a dichotomous DV?

Hello! I ran a logistic regression with a categorical IV (control, low experimental, high experimental) and a dichotomous DV (no/yes). RQ: Were participants in the low or high experimental...

01 February 2020 308 2 View

What is the correct test to run in SPSS to determine the difference between control and treatment groups (paired samples) on four DVs?

The study seeks to examine if the lesson plan in the treatment group is a more efficacious way of teaching the Toulmin model of argumentation (DV1; measured via a quiz on knowledge and application...

11 May 2018 1,036 8 View

How to calculate phase angle, delay and multipath of the signal?

hi everyone, I am trying to understand how to calculate the phase angle, delay, and multipath signal. I hope everyone can help me for ideas. My scenario: I have a radio hardware (SDR) with rx1 and...

12 December 2016 2,169 3 View

What are some unique HPLC column solid phases besides HILIC, divinyl benzene, and diol?

It could be either normal or reverse phase, I am using both.

26 February 2014 9,090 11 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View