I'm currently studying the effects of glucagon in primary hepatocytes but I'm concerned that the media I am using (DMEM 10-1017 CV) is effecting my results. I suppose the real question is what is the most appropriate media that won't influence liver metabolism significantly. Has anyone used Williams E Medium or Ham's F-12K (Kaighn's) Medium? Ideally I want to replicate an environment where glucagon would be released (i.e. low glucose). Any advice would be appreciated.
Thanks,
Sam
Please check
1. Cho CH, Berthiaume F, Tilles AW, Yarmush ML. A New Technique for Primary Hepatocyte Expansion In Vitro. Biotechnology and bioengineering. 2008;101(2):345-356. doi:10.1002/bit.21911.
2. Akie, Thomas E. and Cooper, Marcus P., "Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes" (2015). GSBS Student Publications. 2004. http://escholarship.umassmed.edu/gsbs_sp/2004
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