Sam Man Lee

5 Questions 7 Answers 0 Followers

Questions related from Sam Man Lee

Has anyone used FL83B cells?

I know that most people used HEPG2 or AML12 cells in liver studies but I'm curious why so few labs use FL83Bs (fyi: FL83B: ATCC® CRL-2390™).

06 December 2019 9,087 6 View

What is the appropriate culture medium for studying the effects of glucagon in primary mouse hepatocytes?

I'm currently studying the effects of glucagon in primary hepatocytes but I'm concerned that the media I am using (DMEM 10-1017 CV) is effecting my results. I suppose the real question is what is...

01 September 2018 6,214 1 View

Rats dying suddenly from left anterior descending artery ligation post early surgery. Thought?

Hello everyone, I've been performing LAD ligations on Sprague Dawley rats but a large number of animals have died due to unknown reasons.Mortality only occurs once have finished the surgery and...

26 December 2016 3,326 4 View

Low protein yield / H9C2 cell lysis protocol?

I was wondering whether anyone has worked with H9C2s and could recommend a cell lysis buffer or protocol. This cell-line is still new to me and I am currently optimizing a cell lysate protocol. My...

11 March 2016 6,054 5 View

Is it possible to induce hypoxia to H9c2 cardiomyocytes using a normal incubator?

Hello everyone. I am interested in looking at hypoxia / reperfusion injury in H9c2 cells but there is one issue that is still bothering me. A majority of the papers I have looked at often use use...

13 November 2015 4,842 7 View