What is the antifungal can be used in isolation of bacteria? And how much is the amount?

27 January 2018 0 8K Report

I am going to isolate bacteria from soil. So what is the antifungal can be used in isolation of bacteria? And how much is the amount? I read on nystatin and fungicidin but then which one gives the best result? And how much is the amount? Thank you.

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Nurul Shakirah Ayob's questions See All

1) Does accreditation or background of authors play a role in achieving a closer nutritional value to the established recommended dietary guidelines?

1) Does accreditation or background of authors play a role in achieving a closer nutritional value to the established recommended dietary guidelines for weight loss?

14 June 2024 1,322 0 View

Is it advisable to run reference genes separately from target genes in a comparative Ct analysis on ABI StepOnePlus due to logistical constraints?

Hi Everyone, I'm reaching out for some guidance regarding conducting a comparative Ct analysis with the ABI StepOnePlus system, and I hope you can help shed some light on my dilemma. Here's the...

11 June 2024 4,044 3 View

How to modelling MD of Covalent Inhibitor?

I'm designing a covalent inhibitor on an in silico study. Should I use the "pre-reaction" non-covalent ligand-protein complex or covalently bonded one on the Molecular Dynamic simulation input? I...

20 April 2024 5,662 2 View

Is this FBS debris in my cell media?

Dear all, I prepared my cell media (EMEM+10%FBS) and another cell media (EMEM+2%FBS+1% Pen/Strep). Incubate the aliquot of the media in 37°C for a few days and saw these. They are not moving and...

24 March 2024 7,496 3 View

How to dilute PVA with 31000-50000 Mw in distilled water without agglomeration?

If I stir it at high temperature, I notice a gel like layer at the top, if it wasn't heated, there are 2 layers.

05 March 2024 7,346 2 View

Via Hole Pad does not show in HFSS 3D Layout?

Hi! Why my HFSS 3D Layout doesn't show the via hole pad even when there is trace connected to it?

05 March 2024 8,296 0 View

How to assign port in HFSS 3D Layout?

Hi! How to assign port in HFSS 3D Layout for open-ended design? Can I assign the port on the via holes?

03 March 2024 1,745 0 View

I have found an article in Research gate that my name as an author, unfortunately i never publish that manuscript to anywhere. How to remove?

I have found an article in Research gate that my name as an author, unfortunately i never publish that manuscript to anywhere. How to report to remove the article? attached together is the article.

04 February 2024 9,593 1 View

How to confirm vibration mode from pySCF?

I am working to find the Transition State of a chemical reaction using PySCF. I got that conformation after Opt TS and got exactly 1 imaginary freq. how do I confirm that the negative freq is the...

01 February 2024 8,096 1 View

Mendel crossed peas having round seeds and yellow cotyledons with peas having wrinkled seeds and green cotyledons?

All the F, plants had round seeds with yellow cotyledons. Diagram this cross through the F₂ generation using both the Punnett square and forked-line methods.

12 December 2023 4,319 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View