13 December 2023 1 4K Report

All the F, plants had round seeds with yellow cotyledons. Diagram this cross through the F₂ generation using both the Punnett square and forked-line methods.

More Nurul HUDA Shairil Hafizi's questions See All
1) Does accreditation or background of authors play a role in achieving a closer nutritional value to the established recommended dietary guidelines?

1) Does accreditation or background of authors play a role in achieving a closer nutritional value to the established recommended dietary guidelines for weight loss?

14 June 2024 1,322 0 View

Is it advisable to run reference genes separately from target genes in a comparative Ct analysis on ABI StepOnePlus due to logistical constraints?

Hi Everyone, I'm reaching out for some guidance regarding conducting a comparative Ct analysis with the ABI StepOnePlus system, and I hope you can help shed some light on my dilemma. Here's the...

11 June 2024 4,044 3 View

How to modelling MD of Covalent Inhibitor?

I'm designing a covalent inhibitor on an in silico study. Should I use the "pre-reaction" non-covalent ligand-protein complex or covalently bonded one on the Molecular Dynamic simulation input? I...

20 April 2024 5,662 2 View

Is this FBS debris in my cell media?

Dear all, I prepared my cell media (EMEM+10%FBS) and another cell media (EMEM+2%FBS+1% Pen/Strep). Incubate the aliquot of the media in 37°C for a few days and saw these. They are not moving and...

24 March 2024 7,496 3 View

How to dilute PVA with 31000-50000 Mw in distilled water without agglomeration?

If I stir it at high temperature, I notice a gel like layer at the top, if it wasn't heated, there are 2 layers.

05 March 2024 7,346 2 View

Via Hole Pad does not show in HFSS 3D Layout?

Hi! Why my HFSS 3D Layout doesn't show the via hole pad even when there is trace connected to it?

05 March 2024 8,296 0 View

How to assign port in HFSS 3D Layout?

Hi! How to assign port in HFSS 3D Layout for open-ended design? Can I assign the port on the via holes?

03 March 2024 1,745 0 View

I have found an article in Research gate that my name as an author, unfortunately i never publish that manuscript to anywhere. How to remove?

I have found an article in Research gate that my name as an author, unfortunately i never publish that manuscript to anywhere. How to report to remove the article? attached together is the article.

04 February 2024 9,593 1 View

How to confirm vibration mode from pySCF?

I am working to find the Transition State of a chemical reaction using PySCF. I got that conformation after Opt TS and got exactly 1 imaginary freq. how do I confirm that the negative freq is the...

01 February 2024 8,096 1 View

Is there any explanation for the discrepancy in MTT Assay and Apoptosis Assay observation?

I am conducting a gene modulation on a cell line, which we hypothesize it to mediate the resistance of the cell line towards a certain drug. However, following the gene modulation, MTT assay shows...

23 August 2023 3,813 0 View

Similar questions and discussions
Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,301 7 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

How should I prevent mould contamination on small seeds in a petri dish exposed to warm temperatures?

Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...

24 July 2024 8,850 4 View

How to quantify the fluorescence intensity of only the merge area of an image using ImageJ software?

Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...

22 July 2024 8,770 1 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

What are the key risks associated with foodborne pathogens in imported leafy greens, the impact on public health and effect on regulatory framework?

I am currently writing a systematic review

15 July 2024 9,176 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close