Suning Wang

3 Questions 8 Answers 0 Followers

Questions related from Suning Wang

Extract total RNA: miRNeasy (Qiagen) or mirVana (Ambion)?

I'm planing to purify total RNA from bacteria, and I notice regular RNA extraction kit could only purify RNA > 200 nt. Do you have experience with miRNeasy (Qiagen) and mirVana (Ambion)? Which...

07 July 2013 2,900 6 View

Question about denaturing RNA agarose gel.

I ran my total RNA sample in a denaturing RNA gel (MOPS/formaldehyde gel) and I came across two problems: (1) It was run at 60 V (10 cm gel). The samples are very slow to migrate, ~3 hrs to run...

04 April 2013 6,349 8 View

Northern blot or qPCR

I want to analyze a time-course gene expression after adding some stimulus. For the person who has no experience with Northern blot or qPCR, which method would be easier to learn? What method...

02 February 2013 3,565 2 View