How can I digest the brain tissue in healthy mice as evaluating the apoptosis using flow cytometery by annexin V/propidium iodide (PI)?

We digested the brain tissue with Trypsin ( 1x) for 15 min (all steps were performed in cold conditions) and suspend the cells by pipetting up and down (3-4 times) to the single-cell suspension....

22 August 2020 5,611 4 View

Could anyone suggest a good way to dissolve lard for making a high fat diet?

I am trying to make a ketogenic diet using lard and wonder how to dissolve lard and have a solidify mix of carbohydrate+protein+ lard at the end for 1 litre?

29 May 2017 7,074 3 View

Does anyone have any idea how to inhibit liquid loss inside the capillary tube?

I have two chambers, the inner contain a capillary tube and the outer chamber contains water to maintain the humidity. My problem is that when I fill the capillary tube (by capillary action) and I...

16 September 2015 1,293 7 View

How can I obtain carried charges in a thin film without doing a Hall Experiment?

How can I obtain carried charges in a AlN thin film except doing Hall Experiment method?

12 August 2014 2,130 2 View

Which one is recommended: Treating RNA with DNAase, or treating the cDNA with an RNAase inhibitor?

I want to make cDNA from RNA extractions and I am wondering if it needs to be treated with RNAase or DNAase?

07 May 2014 1,377 20 View

What is the effect of acidic conditions (pH ~4-6) on ascorbic acid quality?

Does anyone know what the effect of acidic condition (acetic acid solution, pH ~4-5.5) on the quality of ascorbic acid (vitamine C) is? How long do you guess that we can keep this solution with...

23 April 2013 3,266 5 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

Sequence of an unknown Oligonucleotide ?

Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .

04 February 2021 5,614 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

Can we use the single-elimination tournament method as the basis of an experiment design?

I want to conduct an experiment in the field of Human-Computer Interaction, where I test the perception of users about which image is better using different camera lenses. In the experiment, I...

18 January 2021 779 2 View

How to get the rupture force vs loading rate plots through SMD simulations from gromacs?

Hi I have force vs time graph and Pulling com positions vs time graphs and got from SMD simulations in gromacs the pulling velocity I am using is constant at 0.01 and the force constant was 1000...

16 January 2021 9,080 5 View