18 March 2014 8 7K Report

I'm trying to work through the protocols for TUNEL staining using In situ Cell Death Detection Kit (Roche), and the recipe of the permeabilization solution keeps bugging me. I admit, we never used sodium citrate in other assays before, especially that of immunohistochemistry, tissue nor cell. We simply added Triton X-100 to PBS in both of these cases. I searched the web and I found a lot of recipes included these particular compounds, but nobody explained why it's there or what it's for. Can anybody shed some light on this matter?

More Yun Li's questions See All
Similar questions and discussions