What is pI value of tev protease?

Mingi Kim @Mingi-Kim-9

23 July 2020 1 7K Report

I've done an anion exchange chromatography of my protein which includes tev protease.

But I cannot find out what is the pI of tev protease

Does anybody know the isoelectric point of tev?

please help me

Edward Michelini

lt calculates out to 8.9 by this analysis tool

https://web.expasy.org/protparam/

but you may want to use the specific mutant in your construct, which often include His tags etc (that typically don't change the pI much). Good luck!

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