Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View

Which software is best for geochemical data analysis and graphics for Geochemical Modelling of Igneous Processes?

I'm familiar with Geochemical data analysis software such as GCDKit, PetroGraph, and Petroplot etc. which one is best and suitable for doing Geochemical Modelling of Igneous Processes? I need...

22 December 2020 9,300 2 View

How to make a high spatial resolution land use land cover map in a 10 year temporal resolution with respect to Indian scenario?

I want to create a large scale land use land cover change map of a village of India in the 10-year interval. Which one is the appropriate satellite image for this. and anybody kindly explains the...

12 October 2020 3,996 6 View

How to calculate new human dose based on In-vivo animal study results?

I have performed In-vivo study in rate model for new formulation as well as marketed formulation. Based on results new formulation have significant improvement in all PK parameters. Oral dose...

20 August 2020 2,209 1 View

What are the fluorescence based experiments to determine interaction of receptor?

i want information on fluorescence based experiments

17 February 2020 1,805 1 View

What can be the measurement scale for perceived employee performance management?

I am doing a research in Cement Industry with emphasis on perceived employee performance management. I am looking for the measuring scale of the same.

09 January 2020 6,363 3 View

How can we extract lactic acid from the biocrude derived from the hydrothermal liquefaction of biomass?

I am trying to separate lactic acid via distillation, from the bio-crude I got after performing hydro-thermal liquefaction of biomass. however, it seemed very challenging as always other organic...

07 January 2020 9,265 1 View

How can I confirm protein expression from Pichia pastoris colonies?

I was successful with the transformation( pPIC9K vector in Pichia pastoris GS115 strain )and got good colonies in RDB plates after 48 hours. later in MD and MM plates also. Can anyone please tell...

09 December 2019 6,346 8 View

Why only Sulpfacetamide is most suitable drug for eye drop preparation not other sulfa drug?

i want to prepare some sulfa drugs ophthalmic formulation

05 December 2019 8,017 2 View

Requesting community to help me to develop a sequence alignment algorithm...?

Hi, I'm working on sequence alignment algorithms. My background is Computer Science. Given two sequences, what could be the max length of a gap and how many insertions/deletion at one stretch I...

16 November 2019 1,767 10 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View