What is the idea of electrophoreses and HPLC?

01 February 2017 2,370 5 View

What are toxic solvents? and If your substrate doesn't dissolve in water what will you do?

toxic solvents

01 February 2017 3,317 0 View

What is the difference between biological and physical decay of complex or labeled compound in animal or human body?

biological and physical decay

01 February 2017 3,653 3 View

What are the target organs that free Iodonium or pertechnetate ions are concentrated in animal or human body?

Free ions

01 February 2017 5,182 2 View

What are the species of iodine that can be formed in the presence of CAT as an oxidizing agent in basic or acidic medium?

Oxidizing Agent like CAT

01 February 2017 3,300 6 View

What are the strong, moderates and weak groups that towards Ortho or Para?

Ortho and Para?

01 February 2017 6,728 8 View

If you have two ortho sites only in ring, then Iodine ion as electrophilic ion will enter in one or two Ortho and Why?

Ortho and Para positions

01 February 2017 7,600 5 View

Why did two different peaks in HPLC technique appear one refer to labeled or complex and the other refer to UV of compound?

Different Rf of complex and its compound

01 February 2017 9,017 1 View

Although, Both nuclear and MR imaging of brain have been studied for decades (since the late 1980s).Explain?

Explain

31 December 2016 3,205 0 View

No title

I need to explain the reponse OK

31 December 2016 6,911 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View