Why did two different peaks in HPLC technique appear one refer to labeled or complex and the other refer to UV of compound?

M. H. Sanad @M_Sanad

02 February 2017 1 9K Report

Different Rf of complex and its compound

Sally Mohamed

I think this because of the difference in the hydrophobicity between them

Badges
Science topic

Similar topics
References

More M. H. Sanad's questions See All

What is meant by mouse strain. in biodistribution study of mice?

mouse strain

05 June 2018 2,445 0 View

What is the idea of electrophoreses and HPLC?

the idea of electrophoreses and HPLC

01 February 2017 2,458 5 View

What is the difference between biological and physical decay of complex or labeled compound in animal or human body?

biological and physical decay

01 February 2017 3,781 3 View

What are toxic solvents? and If your substrate doesn't dissolve in water what will you do?

toxic solvents

01 February 2017 3,401 0 View

What are the target organs that free Iodonium or pertechnetate ions are concentrated in animal or human body?

Free ions

01 February 2017 5,259 2 View

What are the species of iodine that can be formed in the presence of CAT as an oxidizing agent in basic or acidic medium?

Oxidizing Agent like CAT

01 February 2017 3,369 6 View

What are the strong, moderates and weak groups that towards Ortho or Para?

Ortho and Para?

01 February 2017 6,850 8 View

If you have two ortho sites only in ring, then Iodine ion as electrophilic ion will enter in one or two Ortho and Why?

Ortho and Para positions

01 February 2017 7,682 5 View

Although, Both nuclear and MR imaging of brain have been studied for decades (since the late 1980s).Explain?

Explain

31 December 2016 3,276 0 View

How is brain imaging “a relatively new discipline within neuroscience, psychology and medicine”?

I need to explain the reponse OK

31 December 2016 6,984 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View