Can electrophoretic mobility measurements of polymer-coated AuNPs be used to infer the role of charge (if any) in their dispersion stability?

Hello Everyone, We have stable citrate-coated AuNPs dispersed in water. Different polymeric protective coatings (PEG, PVP, BSA) have been chemically grafted to the citrate-coated AuNPs for...

17 November 2020 2,903 16 View

In simple words, what is the difference between Isoelectric Point (IEP) and Point of Zero Charge (PZC)?

There seems to be a lot of arbitrary and sometimes contradictory explanations for the difference between the Isoelectric Point (IEP) and the Point of Zero Charge (PZC). Other times, the two terms...

15 February 2020 6,617 9 View

What is the difference between a stabilizing agent (stabilizer) and a dispersing agent (dispersant) in a colloidal dispersion?

Can these two terms be used interchangeably as commonly seen in the literature? Under which category are surfactant and polymer additives or coatings classified when used in colloidal...

23 August 2019 1,833 6 View

Can we have solid bridges forming between nanoparticles in aqueous environments?

Solid bridging (e.g., due to ambient-temperature sintering and solid-solid diffusion) often occurs between dried nanoparticles. My questions is: Upon the "physical" contact between two...

21 August 2019 3,779 6 View

Is it possible to define stream-function in 3D flow? If so, what is the physical meaning of stream-function in 3D?

I am aware that several definitions of stream-function in 3D flow do exist in the literature. But I am at a loss on what stream-function physically means in 3D.

10 May 2019 5,571 6 View

When defining concrete damage in ABQUS using SI(mm) unites the entering values of the strain should be in (strain) or micro strain ?

When defining concrete damage in ABQUS using SI(mm) unites the entering values of the strain should be in (strain) or micro strain ?

14 April 2019 9,247 6 View

In the laminar flow of a nanofluid with a liquid base fluid, can we use Knudsen number to show that the liquid phase can be treated as a continuum?

If so, how can we define the Knudsen number for solid nanoparticles dispersed in a liquid carrier? And how can the mean free path of liquid molecules be calculated? Conventionally, Knudesn number...

30 March 2019 8,265 28 View

Does nanotechnology harbour any risks? What is the relationship hazard, exposure and risk in toxicology of nanomaterials?

I'm looking for Does nanotechnology harbour any risks? What is the relationship hazard, exposure and risk in toxicology of nanomaterials?

21 March 2019 9,295 8 View

Do nanoparticles acquire a surface charge when suspended in pure water?

If the answer is yes, then does the charge acquisition mechanism change with respect to metallic, metal oxide, and carbon-based nanoparticles? And would it also change between totally pure water...

13 February 2019 6,698 5 View

How to define the epoxy adhesive accurately into the abaqus program ?

i am working on abaqus program to model reinforced concrete beam strengthened by near surface mounted fiber , i need to know how define the epoxy accurately

24 December 2018 6,102 8 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Is nr blast important?

Hello, I have designed a specific primer for my Gene, when I blast this primer, it just finds the mRNA of the gene, but in nr blast, I have found some CDSs that are not relevant to my Gene. Do...

05 February 2021 5,842 3 View

Multiple insertions with Q5 Site-directed mutagenesis?

03 February 2021 221 2 View

How are wild-type and mutant gene and protein isolations and analysis performed?

Considering that we hypothetically designed an experiment, we have an x gene, and we have the mutant version of this gene with a single nucleotide mutation in one of the exons. What kind of an...

22 January 2021 9,589 12 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

How to calculate percent of identity in aligned RNA sequences with a sliding window ?

Hello, I aligned some RNA sequences to identify if some part are similar. I would like to represent my result with the similarity in y-axis and the alignement in x-axis. I want to calculate this...

13 January 2021 7,833 3 View

How to design reverse primer with ambiguous nucleotides?

How to design, reverse primer with ambiguous nucleotides?

03 January 2021 6,762 4 View

Experimental Data Set of Sluice Gates Under Free Flow Regime?

Could anybody share a dataset for the sluice gates operating under the free-flow regime? I need the upstream and downstream depths as well as the opening of the sluice gate at least. Any help...

17 December 2020 4,405 1 View

Problem executing MrBayes. Help?

Hi, I'm trying to run MrBayes 3.2.7 with a matrix of 37 species and 20561 nucleotides. I consistently receive the following error: MrBayes > Execute...

18 November 2020 3,867 3 View

How can I apply degenerate primer in SerialCloner (2.6.1) to run a PCR?

I have to carry out an in silico PCR on the 16S-rRNA gene of 5 random bacteria strains. I would like to evaluate the resulting amplicon of different primer pairs. Because I can only use free...

14 November 2020 2,341 2 View