Stromal cell lines are derived from the core of tissues, and used in various experiments in place of primary cells.
repeated adherent-culture , the stromal cells will overgrowth rather the bone marrow cells
I am not sure what the question is you are asking.
Stromal cells from the bone marrow are the adherent cells (stick to plastic) from e.g. a bone marrow aspirate. They are primary cells (as are any non-cancerous cells taken from an organism and placed into culture).
Since 2006 (Dominici et al 2006) adherent cells from the bone marrow (or elsewhere) must meet a minimum criteria for the expression of certain markers (such as CD90 expression) to be classed as Mesenchymal stromal cells (MSC).
https://phpsistemas.dti.ufv.br/noticia/files/anexos/phpCDlYcY_5654.pdf
One criteria for a cell to be a MSC is that it should not express certain haemopoetic markers whereas the majority of cells form the bone marrow will have these markers (see paper link above) .
"MSC must express CD105, CD73 and CD90, and lack expression of CD45, CD34, CD14 orCD11b, CD79a or CD19 and HLA-DR surface molecules"
Hope this helps
Gary
@ Gary Morley :
Thanks for such a nice explanation.
I know that Stromal cells are primary cells and adherent to surface.
But recently i found few papers where workers have developed a method to subculture these cells like cell lines. and similar type of propagating cell culture has also been reported from spleen by various groups.
I was curious whether this method is exclusive to spleen and bone marrow or can be applied to other primary PMN cells too..Thats why i asked how does stromal cells (of any origin) differ from primary cells (of same origin)..
Oh I see - MSC can be isolated from a variety of tissues such as adipose and synovial cells too. Any cell that fulfils that criteria from that 2006 paper can be considered to be an MSC I believe (marker expression and the ability to differentiate into a number of cell types). The current thinking is that they arise from pericytes (small cells that attach to blood vessels and that can be released during trauma). MSC can be sub-cultured but unless they have been immortalised (using e.g. hTERT) then they have a finite expansion capability in culture (~20 passages depending on age of donor). Obviously they are under a lot of investigation in clinical trials as they secrete a broad range of growth factors/cytokines etc. that are believed to participate in healing. However in the long term I can see clinical work moving from MSC towards disease treatment with the exosomes that these cells release (non-cell based treatments are viewed as safer).
It is probably worth mentioning that MSC are almost identical to fibroblast cells in everything except for the secretory molecules that they release (see: Blasi et al. Vascular Cell 2011, 3:5).
Anyway, good luck!
Gary
I think you mean the difference between culturing BM-MSCs and BM stroma. The essential difference is culture medium, where primary Bm-MSCs usually grew in DMEM-low glucose supplemented with 11% heat-inactivated FBS with l-glutamine. Stromal cell culture is based on long term culture medium that composed of IMDM containing 10% FBS 10% horse serum and hydrocortisone. Stromal culture usually used in co-culture with HSCs or used to maintain MSCs for a long time in-vitro.
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