How to remove unedited version of the manuscripts from research gate?

Our manuscript was published ahead of print. The edited and revised version is now available. How do I remove the old version of the manuscript from the research gate?

01 February 2021 5,965 1 View

What causes up shift of protein band in Western Blot?

Hello, I am working on a recombinant protein expressed in the form of inclusion bodies for my bachelor thesis. After solubilization and refolding I performed an SDS-PAGE and to confirm the...

21 October 2020 5,311 8 View

How to prevent false negative FISH results?

I am analyzing data from a FISH experiment and notice that there are false negatives. What is the best option to change in the experimental set up to avoid this from happening?

04 October 2020 5,777 1 View

Can you suggest an academic platform (in addition to RG and Academia) where PhD students can share drafts of their papers for feedback?

I am looking for a platform where PhD students would feel comfortable to share their papers at a very early stage of research and could receive a helpful feedback.

05 August 2020 4,713 3 View

Difficulty in interpreting data processed with two-way ANOVA?

I have a two-factor experiment. We investigated the effect of the drug on the level of erythrocytes after surgery. One of the factors is the presence of the operation, the second is the presence...

22 July 2020 7,687 5 View

Do you have information about the available free program for determining the area of leaves using a photo or image scanner?

21 June 2020 2,297 5 View

Please, help. Should one or two-way ANOVA be used in case of determining the drug effect of the drug against the background of the model operation?

We studied the effect of a biological supplement on biochemical and hematological parameters rabbits with and without model operation. I have 4 group of rabbits (n=8) and 3 time points (before...

15 May 2020 5,846 17 View

Does anyone know of a free biology software NOT SUBJECT TO COPYRIGHT for publishing papers?

Does anyone know of a free biology software NOT SUBJECT TO COPYRIGHT for publishing papers?

24 February 2020 7,748 3 View

How to analyse measurements from 3 different conditions?

I have cytokine measurements from 3 different conditions: What is the best way of comparing the differences between the conditions ? Many thanks Irina

20 February 2020 5,750 3 View

Possible problems with eNOS intracellular staining in circulating endothelial cells?

I am trying to set up a protocol to study circulating endothelial cells by flow cytometry. First approach was to identify CD45-CD146+ population and analyze expression of cell adhesion molecules...

28 January 2020 9,190 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Is it possible, for a limited dateset of images, to use Convolution filters + Random Forest instead of UNet?

UNet is 'relatively' recent and was invented mainly to deal with biomedical images, yet is it also possible, for a limited dateset of images, to use Convolution filters + Random Forest instead of...

21 February 2021 4,858 3 View