What is the basis of the ratio for sample to reagent frap?

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What is the most efficient method to determine the functionalities on a silane-treated glass surface?

We have recorded the FT-IR spectrum of silane-treated* glass fibers that we bought from a supplier and wish to determine the functionalities on the surface of our glass fibers using the FT-IR data...

04 March 2024 2,309 1 View

What factors could cause both storage and loss moduli to rise during a strain sweep measurement?

Strain amplitude sweeps are standard rheological measurements to determine a polymer's linear viscoelastic region. My system of interest is a filled, reactive thermoplastic polymer.

29 November 2023 1,074 5 View

DNAase RNAase free reagents like BSA?

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What is common method of ATP binding assay?

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The results obtained from the analysis of my study show that the average and SNR are different, ?

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X in stock solutions,syber?

Iwant to know have anyone any experience about making 1000x sybergreen from it powder?

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Request for an instrument?

can anyone help me to access EPQ (forsyth's 1980 ethics position questionary)?or can mail the questionary for me?

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My SDS-PAGE gel has smear. can you help me to resolve the problem? the samples are protein(28KDa)?

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How can i make an 10x ammonium buffer for pcr ?

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Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

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06 August 2024 9,710 1 View

Interested in a SCOPUS collaboration?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

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28 July 2024 8,027 3 View

Can sulfuric acid be used to prepare anthrone reagent when it has oxidized and changed color in reaction to light?

Sulfuric acid has changed color to yellow.

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Is the protecting group boc of the amino group stable at 37°C?

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Can anyone share immunostaining protocol for human cells?

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How could I determine antioxidant activity of Capsicum annuum fruits?

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Karen A. Darbinyan

The sample/reagent ratio 1:15 to 1:30 is the linear range for measurement this is not so critical but it should be standardized across all samples and calibrators and you need to use the same ratio which suitable for you for all analyses.

Bruno Luis Ferreira

Darbinyan is right! The most important is to follow the same pattern to perform all your analyses.

Niloofar Ghobadi

Dear all

thanks a lot for your valuable answers.