Please how can I justify my regression results?

I conducted a study to investigate stock market reaction to equity issues announcement and i ended up with 64 announcements and i then used the announcement day returns as my DV and investigated...

14 January 2021 1,127 3 View

How to correctly indicate a zinc finger in a protein for molecular dynamics?

I am preparing a protein that has a Zn finger for molecular dynamics. To indicate the finger of Zn a patch is placed in which the deprotonation of the cysteines is necessary so that the Zn ion can...

05 March 2020 1,425 3 View

No title

28 February 2020 1,633 2 View

How do I calculate solar absorbance and thermal emittance of absorber coating from reflectance spectrum using standard AM 1.5 and blackbody radiation?

I have a reflectance data of my solar absorber coating from UV-Vis, I want to calculate the solar absorbance of my coating using standard reference spectrum AM 1.5 and thermal emittance of thesame...

15 November 2019 225 3 View

How can Iimprove the resolution on a GC?

When I run a O2 and N2 standard in the gas chromatograph, the peaks are overlapped, How can I improve the resolution of the chromatogram. This situation is new, last monththe calibrationwent just...

22 August 2019 4,050 6 View

On a EVO LS10 SEM, I can see images on spot 0 of the aperture, however on spot 1 and 2, I cant see any images. what could be responsible for this?

I an using the EVO LS10 SEM from Zeiss, I can see images on spot 0 of the aperture, however when the aperture is switched to spot 1 or 2, I am unable to see any image. Please what could be...

05 February 2019 3,902 1 View

Where to get yeast W303-K6001 strain?

15 September 2018 3,735 2 View

My solvent-casted PVDF film was opaque and somehow shrunk during vacuum drying. What should I do?

I'm trying to make smooth PVDF film using solvent casting dried under vacuum from 20 mL of 15% PVDF in DMF solution. The vacuum dryer temperature was at 55 deg C. After some time, this is what I...

29 January 2018 803 4 View

How can I get rid of the polysaccharides on orchids tubers to isolate DNA?

I try to isolate DNA from orchids tubers, the problem is they become kind of gelatinous because the polysaccharides.... so I want to get rid of them. Thank you!

18 September 2017 9,815 3 View

Shuold the "Reads" be included or taken into consideration for calculating the researcher's score in RG?

Any RG's member

02 March 2017 6,338 8 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

How to dissolve querceitin in HEPES?

I am trying to do the isothermal titration calorimetry study of quercetin. I want to reach a final concentration of 5 mM quercetin in HEPES or MES buffer with

15 February 2021 2,159 3 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

How to measure Tg (Glass Transition) in lyophilised sugar/protein matrices using DSC?

I have been attempting to measure the glass transition temperature of various amorphous powders consisting of sugar/protein mixtures and have had little luck. I typically use a scanning rate of...

13 January 2021 10,022 3 View

The saturation point does not reach in my ITC. Does this indicate that the interaction is weak or there is no interaction?

21 December 2020 9,407 8 View

How to dissolve peptide in IxPBS?

Hello! I am working with a peptide of 19 amino acid composition. It has 7 positive charged residues (Arg + Lys) and no negative charged residues so it's obvious the peptide is basic. I tried...

16 November 2020 1,606 7 View

How to correct a deviation in the molar ratio in an Isothermal Titration Calorimetry equipment?

Our equipment of ITC does not reach the molar ratio (N=1) of a titration of EDTA with Ca2+ in MES buffer pH 6. We have cleaned with DCN 90 and HCl pH 2, but the N value is very low, around...

10 November 2020 3,137 3 View

How can I maintain a surface at isothermal condition, say at 300 deg Celsius.?

I have 6 heaters each of power 0.5 kW and mounted below a curved mild steel sheet of thickness 4 mm. The heaters and the below section is covered and insulated. I want the top surface to be...

05 November 2020 199 5 View

No title

26 July 2020 1,511 0 View

How can we remove thermal history of a polymer on DSC without affecting the solid state of the active ingredient incorporated within?

I am actually working on pharmaceutical formulations with thermoplastic polymers such as "copovidone or Kollidon VA64 and active ingredients like "Paracetamol". When i was running DSC with pure...

01 March 2020 4,072 5 View