I am presently working on modification of carrier vehicles to be used for physiological systems. Peptide conjugation is required for this to achieve a targeted delivery system. Kindly suggest some useful insights.
Activate the carboxylic group with standard carbodiimide chemistry (e.g. EDC-HCl + NHS, if possible slightly acidic conditions), then add your peptide. If you have more than one amino group, consider deprotecting the synthetic peptide after coupling, i.e. if possible, have the whole synthesis done at the peptide facility.
Standard EDC+NHS chemistry is a pain in the ass as hydrolysis is always an issue. Use carbodiimide chemistry in organic solvent if possible for better results.