What test should I use?

15 February 2021 911 5 View

Why am I not getting colonies using the Agilent QuikChange II kit?

Hello everyone, I hope you are very well. I am performing site-directed mutations with the QuikChange II kit. My first ones have a length of 50bp and a Tm of between 75 and 81 approximately. I...

09 November 2020 242 2 View

Repurposing a published image for a thesis - permission requiered?

I would like to use a crystal structure of the heme group from PDB for my thesis, for the introductory topic of cofactors (Figure 2, 10.1111/j.1432-1033.2004.04411.x ). The published figure has...

19 August 2020 1,798 3 View

How to convert .txt into plink files (ped/map or bed/bim/fam)?

I have a huge oncoarray_genotype_XXXX.txt file with 11.7 GB size. I would like to convert this file into adequate files for analyzing in plink software. However, I could not find how to conduct...

08 July 2020 9,302 2 View

Using RPMI vitamin solution for Bacteria growth?

I am searching for a solution containing both essential and non-essential amino acids. Mammalian cell culture seems to use these quite often, I would like to know if someone has experience using...

16 March 2020 7,696 3 View

Flow cytometry multicolor panel builder software tool that scans all commercial antibody databases?

Does anyone know of a web app or software for designing polychromatic flow cytometric panels that's able to scan all commercially available primary conjugated flow cytometry antibodies (i.e. from...

13 December 2019 7,047 4 View

What concentration do you use to elute a NiNTA resin?

The protocol of Thermo Scientific NiNTA resin (cat. 88221) for purification of proteins containing a hexahistidine tag use an elution buffer containing 250 mM imidazole. For fun after this elution...

01 August 2019 4,271 3 View

Dehydrogenase enzyme activity: would you take a look at my data?

My experiment involve a dehydrogenase and I would like to identify the optimal substrate. For this an assay was established based on the use of DCPIP as cofactor, which when reduced the absorbance...

11 January 2019 2,476 5 View

Is one gRNA enough to delete a region by CRISPR/Cas9, whose HRs are ~1kb apart?

Dear all, Me and a colleague have come across the question whether a single guide RNA is enough to sufficiently delete e region (let's say a complete KO), for which the two homologous regions are...

26 September 2018 5,318 3 View

Can I use RNA protect for RNA bacterial extraction inoculated on food matrix, and after that, dilute it?

Hi people! I would like to ask whether anyone of you have extracted RNA from bacteria on a food matrix. I have to inoculate bacteria on nuts, and after my processing tests, I need to extract RNA...

11 September 2018 7,343 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View