I am looking for the best marker/s for isolating NKT cells from spleen and liver of CBA mice. Plus, what is the best geting strategy to analyze the cells?
the reason of decreased level of IL5 in patient wit OAB
13 May 2023 6,829 2 View
My SWATCUP unable to accept more than 18 parameters for sensitivity analysis? Is there any one who have solution in this regard?
23 March 2022 850 1 View
hello everyone i am very interested on modeling and simulation of rock blasting( bench blasting) particularly, analysis of crack/fracture growth during blasting. i use this ls-dyna software for...
13 February 2022 3,481 2 View
I went to correlate field soil moisture data with satellite soil moisture data of my study watershed for a period between June 2021 to May 2022. But i couldn't know the exact satellite overpass...
16 May 2021 4,751 3 View
What are the pros and cons of sharing preprints of academic researches that did not pass peer-reviewing? Since we all know the benefit of peer-reviewing in academic researches and scientific...
09 December 2020 1,587 3 View
I am working on mouse liver lymphocytes to study the immune response against schistosmiasis but I am having difficulty getting pure single cell suspension because the mice that I am now working...
08 February 2017 6,117 1 View
Immunologists
22 December 2015 4,818 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View